Our Services

We specialize in devising strategies for reliable, reproducible, and high quality data collection

Services we offer

Sample Processing and Cryopreservation

  • Processing of venous blood for isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs)
  • Bone marrow aspirate processing and storage

Epitope Discovery

  • Enzyme-Linked ImmunoSpot (ELISPOT) assay utilized in the identification of T cell epitopes

Multiparametric Flow cytometry

  • Phenotypic characterization of cells or their activation, proliferation or antigen experience profile.
  • Functional characterization of cells by Intracellular Cytokine Staining (ICS), CarboxyFluorescein Succinimidyl Ester (CFSE) staining or other proliferative assays, Killing assays, PhosphoFlow, Basophil activation test (BAT), Cell cycling, etc.

Serological Assays

  • MAGPIX Luminex assays used in the identification and quantification of an array of proteins in culture

Study design

  • GLP certified facility that abides by GLP guidelines and regulations and can cater to pre-clinical as well as Phase I – III clinical studies.

Next Generation Sequencing

  • Certified SomaLogic service provider capable of targeted and custom sequencing of both DNA and RNA samples including T-Cell Receptor (TCR) and B-Cell Receptor (BCR) sequencing

Histology

  • Processing, embedding, sectioning and staining of formaldehyde-fixed and cryopreserved tissues using standard histological stains, immunohistochemical stains or custom developed stains.

 We offer a range of assays and furthermore we are able to design customized assays specifically tailored to address your research niche.

Overview of Assays

The core of HITS is a dedicated 4-laser LSRII flow cytometer equipped with a high-throughput sample loader and a Biomek NX robotic workstation.

Phenotypic characterization

  • T cells, T regs, B cells, Monocytes, NK cells, ILCs, DCs, MDSCs
  • Immune-checkpoint receptors
  • Tetramer analyses

Functional and proliferative profiling

  • Intracellular cytokine stain (ICS)
  • Basophil activation test (BAT)
  • CFSE dilution assay
  • Cell cycling
  • Phospho Flow cytometry
  • Killing assays

ELISPOT

  • Epitope Mapping
  • Functional profiling

Serological Assays

  • ELISA
  • Luminex MAGPIX

FRET (Fluoresence Resonance Energy Transfer)

  • Protein interaction studies

T cell and B cell expansions

  • Using feeder cells or specifically designed APCs we can enhance your primary T or B cell lines

DNA and RNA Analyses

  • Microarray testing
  • Genotyping
  • Next Generation Sequencing
  • Bioinformatics Services

Histology

  • Processing/embedding
  • Sectioning
  • Routine staining
  • Specialized staining
  • Immunohistochemistry
  • Cryosectioning